Roger Castells-Graells

Roger Castells-Graells

Postdoctoral Researcher

University of California Los Angeles

About me

I am a biotechnologist and biochemist with over 10 years of research experience. I am currently a postdoctoral researcher in the lab of Prof. Todd Yeates at the University of California Los Angeles (UCLA), where I combine protein design with structural biology to (1) develop new imaging tools to study small proteins (<50 kDa) with cryo-electron microscopy (cryo-EM) using designed protein cages as scaffolds and to (2) design new protein assemblies and nanomachines for biomedical applications using AI-based computational tools. My research interests lie at the interface of structural, molecular, cellular, computational and synthetic biology. I received my undergraduate degree in Biotechnology from the Autonomous University of Barcelona (Spain) in 2015, and earned a PhD in Biochemistry from the John Innes Centre (UK) in 2019 under the mentorship of Prof. George Lomonossoff. During my doctoral training, I investigated the details of the dynamic conformational changes that occur during the maturation cycle of a eukaryotic virus, using high-resolution cryo-EM.

Interests
  • Biochemistry
  • Structural Biology
  • Synthetic Biology - Protein Design
  • AI - Deep Learning
  • Virology
Education
  • PhD in Biochemistry, 2019

    John Innes Centre (UK)

  • BSc in Biotechnology, 2015

    Auntonomous University of Barcelona (Spain)

Featured Publications and Preprints

Full list of publications –>

Cryo-EM structure determination of small therapeutic protein targets at 3 Å-resolution using a rigid imaging scaffold
Cryoelectron microscopy (Cryo-EM) has enabled structural determination of proteins larger than about 50 kDa, including many intractable …
Cryo-EM structure determination of small therapeutic protein targets at 3 Å-resolution using a rigid imaging scaffold
Design of Beta-2 Microglobulin Adsorbent Protein Nanoparticles
Beta-2 microglobulin (B2M) is an immune system protein that is found on the surface of all nucleated human cells. B2M is naturally shed …
Design of Beta-2 Microglobulin Adsorbent Protein Nanoparticles
Plant-expressed virus-like particles reveal the intricate maturation process of a eukaryotic virus
Many virus capsids undergo exquisitely choreographed maturation processes in their host cells to produce infectious virions, and these …
Plant-expressed virus-like particles reveal the intricate maturation process of a eukaryotic virus

Experience

 
 
 
 
 
University of California Los Angeles (UCLA)
Postdoctoral Researcher
University of California Los Angeles (UCLA)
January 2020 – Present California, US
Computational design and characterization of novel protein assemblies as imaging scaffolds for structural biology (cryo-EM) and de novo design of protein assemblies and nanomachines for biomedical applications (using AI-based computational tools) in the lab of Prof. Todd Yeates.
 
 
 
 
 
John Innes Centre (JIC)
Postdoctoral Researcher
John Innes Centre (JIC)
October 2019 – January 2020 UK
Study and production of virus-like particles for biotechnology applications like vaccines. Horizon 2020 Pharma Factory project in the lab of Prof. George Lomonossoff.
 
 
 
 
 
John Innes Centre (JIC)
PhD Student - John Innes Foundation Fellowship
John Innes Centre (JIC)
October 2015 – September 2019 UK
-PhD Project: Study of virus maturation using transient expression systems and biophysical and structural methods, including cryo-EM, in the lab of Prof. George Lomonossoff (PhD project, John Innes Centre, June 2016 - September 2019).
-Thesis: Viruses in motion - maturation of an insect virus-like particle – a nanomachine
-Collaborations with:
• Prof. Jack Johnson (The Scripps Research Institute, USA)
• Prof. Neil Ranson (The Astbury Centre for Structural Molecular Biology, UK)
• Prof. Tatiana Domitrovic (Universidade Federal do Rio de Janeiro, Brazil).
-Rotation Projects:
• Study of the alteration of host vesicular trafficking by an effector from Phytophthora infestans and study of the deletion of a gene cluster in tomato with CRISPR/Cas9 in the lab of Prof. Sophien Kamoun (Rotation 3, The Sainsbury Laboratory, March - May 2016).
• Study of starch synthesis in wheat grains in the lab of Prof. Alison Smith (Rotation 2, John Innes Centre, January - March 2016).
• Production of engineered mosaic virus-like particles from turnip crinkle virus (TCV) in plants in the lab of Prof. George Lomonossoff (Rotation 1, John Innes Centre, October - December 2015).
 
 
 
 
 
Centre for Research in Agricultural Genomics (CRAG)
Research Intern
Centre for Research in Agricultural Genomics (CRAG)
October 2014 – February 2015 Spain
Study of the structure of plant genomes looking at elements like transposons with bioinformatic tools. Mentored by Dr. Cristina Vives, Prof. Pere Puigdomènech and Prof. Josep M. Casacuberta.
 
 
 
 
 
Ludwig-Maximilians Universität München (LMU)
Research Intern - Amgen Scholars Fellowship
Ludwig-Maximilians Universität München (LMU)
July 2014 – September 2014 Germany
Study of chloroplast membrane proteins, OEP21 and Tic-22, with Dr. Bettina Bölter in the lab of Prof. Jürgen Soll.
 
 
 
 
 
University of Zurich (UZH)
Research Intern - BUSS Fellowship
University of Zurich (UZH)
July 2013 – August 2013 Switzerland
Study of the durable disease resistance gene Lr34 from wheat with Dr. Simon Krattinger in the lab of Prof. Beat Keller.